مشخصات پژوهش

Recognizing genetic diversity and classifying the Germplasm is one of important activities in race improvement and management of genetic herbaceous sources. In this research random amplified polymorphic DNA (RAPD) &inter-simple sequence repeat (ISSR) to be used for surveying genetic varieties of 13 masses of Zataria multiflora. The leaf of thyme from 13 different regions in Kerman was collected. The extraction of DNA from the leaf was done by cetyltrimethylammonium bromide(CTAB) method with a little change. The quantity and quality of extracted DNA was measured by electrophoresis equipment & spectrophotometer. 10 markers of RAPD and 10 markers of ISSR which had produced the clearer bands in polymerase chain reaction (PCR) were used for analysis. by considering of the results, the markers were produced in the total of 179 polymorphism of 212 bands by percent of polymorphism of 84.4. 13selected regions in 3 separated groups that the most genetic similarity due to the similarity the coefficient of Dice by NTSYS software among the Bluck masses and Jiroft was 89.20% and the less similarity was considered among Bluck masses with Bam (52.5%). created cluster was had harmony with geographical conditions. 2dimensional graph resulting from the Principle component analysis(PCA) nearly was accordance with dendrogram due to Unweighted Pair Group Method using arithmetic Averages(UPGMA) method and cophenetic correlation coefficient was 77% in this research. the results of this research showed that markers RAPD & ISSR could be a useful tool for surveying genetic varieties of herbaceous samples and help for amendments of future researches & management of Zatraia multiflora germplasm.