University of Jiroft | Naser Askari

Research

Title	Negative hydrostatic pressure is an unnoticed but significant source of contamination in tissue culture
Type	Article
Keywords	lily, tissue culture, in vitrro
Researchers	Naser Askari, Geert-Jan De Klerk

Abstract

Plants are characterized by a negative hydrostatic pressure, brought about by transpiration and by capillary activity of xylem vessels (Taiz and Zeiger, 2010). Because of this, a stem that is being cut sucks up what is nearby. Often this is air but it may also be liquid. The diameter of the xylem vessels is 50-100 μm, so when the liquid contains bacteria (that are typically