November 23, 2024
Amirreza Amirmijani

Amirreza Amirmijani

Academic rank: Assistant professor
Address: 8km of Jiroft-Bandar Abbas Road, University of Jiroft, Jiroft, Iran
Education: PhD. in قارچشناسی
Phone: 03443347070
Faculty:

Research

Title
Isolation and identification of fungi associated with dried fruits of white mulberry
Type Presentation
Keywords
ITS, fungal species, morphology, Morus alba, Jiroft
Researchers Amirreza Amirmijani, mahdiyeh sadeghian

Abstract

This study was undertaken to investigate on fungal species associated with dried fruits of Morus alba. During 2016, a total of 25 samples, were collected from common markets in south of Kerman province, Jiroft. After surface disinfestation with 0.5% NaOCl, small fragments of dried fruits were placed onto potato dextrose agar (PDA). Fungal isolates were purified by single spore method. Morphological characteristics such as colony color, shape and size of hypha, conidia and conidiophores were recorded. In addition, for confirmation of morphological identification, genomic DNA was extracted from mycelia by the chelex method. The rDNA ITS region (including 5.8S rDNA) was amplified by a polymerase chain reaction using ITS1 and ITS4 primers. Based on morphological and molecular data, five species including Alternaria alternata, A. tenuissima, Arthrinium arundinis, Cladosporium cladosporioides and Fusarium oxysporum were identified. To the best of our knowledge, this is the first report of fungi associated with dried fruits of white mulberry in Iran.