Amirreza Amirmijani

In a survey of tropical plant diseases in southern and southeastern Iran, samples of diseased Mangifera indica and Psidium guava leaves with necrotic symptoms were collected between 2021 and 2022. Six representative isolates of Neopestalotiopsis and Robillarda (three isolates for each) were studied using morphological characteristics as well as multi-locus phylogenetic analysis based on (i) the internal transcribed spacer (ITS) region of the nuclear rDNA, (ii) part of the translation elongation factor 1-alpha (tef1), and (iii) the β-tubulin (tub2). After morphological investigation, our phylogenetic analysis revealed that the Neopestalotiopsis and Robillarda isolates under study differed from all previously described species within these genera. Based on our polyphasic approach, two new species, including Neopestalotiopsis guavasp. nov. from necrotic Mangifera indica and Robillarda khodaparastiisp. nov. from Psidium guava are described and illustrated from Iran.