Fusarium root and crown rot caused by Fusarium oxysporum f. sp. radicis-lycopersici (FORL) is one of the most important tomato diseases in the South of Iran, causing large economic losses. The management of this disease is primarily accomplished through the utilization of cultivars that possess resistance to the pathogen and by implementing a system of crop rotation. The present investigation endeavors to assess the response of four common and commercial tomato varieties, namely Sancid 6189, Basimo, Afra, and 8320, under greenhouse conditions in Hormozgan province to FORL. In order to achieve this objective, various parameters were measured, including the disease index as well as growth characteristics such as fresh and dry root weight, and root length. The findings indicate that Afra and Snancid 6189 exhibit the highest levels of resistance and sensitivity, respectively. Other cultivars manifest diverse degrees of susceptibility. These empirical outcomes hold substantial potential for application in the context of disease management and control programs.