zahra roudbari

The aquarium fish industry has become a capacity for job creation and income generation. Aquarium fish were used for decoration and are creating tranquility in the environment and beautification in public places. This study investigated genetic variation between different populations of various varieties of guppy ornamental aquarium fish using inter simple sequence repeat (ISSR) markers in Iran for the first time. DNA was extracted from twelve strains. The polymerase chain reaction was performed using seven ISSR primers. GenAlex 6.501 and XLSTAT software were used to analyze data. Results of analysis of molecular variance showed that 54% of observed polymorphism belongs to variance among populations, and 46% corresponds to diversity between populations. The highest and the lowest polymorphism percentage belonged to (GAG)5GC (90%) and (AG)8C (41%) markers, respectively. The percentage of polymorphic loci ranges from 32.31% to 86.15%. The results of the phylogenetic tree showed that this tree was divided into two major clusters. Cluster I included only two populations and cluster II contained the other ten studied populations. It is suggested that this marker system was efficient in discriminating each genotype at the molecular level and can be used for genetic diversity analysis for aquarium guppy fish because a greater understanding of the species' potential is necessary for supporting long-term genetic improvement.