آتنا نعیمی

A novel bio-nanocomposite was developed by incorporating the extracted nanochitosan from shrimp wastes with Schiff base cobalt complex (Chit–Co complex). The phytosynthesis of Chit–Co complex/Ag bio-nanocomposite was designed utilizing Chit–Co complex at the presence of Ferulago angulate extraction and characterized by AFM, SEM, EDAX, TEM, FT-IR, and elemental analysis. The radioprotective application of this bio-nanocomposite on human lymphocyte cells was evaluated using micronucleus (MN) assay. Total antioxidant activities of it were evaluated using FRAP and DPPH assays. Chit–Co complex/ Ag bio-nanocomposite significantly decreased the frequency of micronuclei in human lymphocytes exposed to ionization irradiation (IR). The highest protection was observed at 200 μg/ml. Also, maximum antioxidant activities of bionanocomposite were provided at the same dose. These data exhibit the radioprotective effect of a bio-nanocomposite based on wastes of living organisms can be an excellent radioprotective agent, which can protect the normal cells of human against the genetic damage by IR.