In study, CuO nanoparticles has been synthesized using Spinaciaoleracea leaves extract as a green,
renewable and non-toxic reducing agent and stabilizer in the absence of hazardous and toxic solvent
and materials. XRD and TEM have been used for the characterization of biosynthesized nanoparticles.
The crystalline nature CuO nanoparticles was determined by XRD analysis. The spherical CuO
nanoparticles with size in the 15 nm have been formed, as shown by TEM image.