In the present study, Fe3O4 magnetic nanoparticles (MNPs) are one of the most extensively investigated inorganic nanomaterials for biomedical applications due to their special superparamagnetic ability and outstanding biocompatibility. In magnetic resonance imaging (MRI), MNPs are commonly utilized as contrast agents for disease diagnosis. Combining multiple imaging modalities is currently being viewed as a new trend in disease diagnosis and therapy, with the potential to raise disease diagnosis accuracy while also providing novel therapeutic strategies. New contrast agents for multifunctional imaging are therefore desperately needed. Fe3O4 MNPs are being considered as a good candidate for use in the development of a multifunctional platform for disease diagnostics and therapy. We report successful eco-friendly syntheses of Fe3O4 nanoparticles with a size of 25.7 nm via a low-temperature route (Figure 1). According to EDAX's detailed analysis, the generated samples include 62.6% wt of Fe and 39.4% wt of O.