02 آذر 1403
اميررضا اميرميجاني

امیررضا امیرمیجانی

مرتبه علمی: استادیار
نشانی: جیرفت، کیلومتر 8 جاده بندرعباس دانشگاه جیرفت
تحصیلات: دکترای تخصصی / قارچشناسی
تلفن: 03443347070
دانشکده: دانشکده کشاورزی

مشخصات پژوهش

عنوان
Isolation and identification of fungi associated with dried fruits of white mulberry
نوع پژوهش مقاله ارائه شده
کلیدواژه‌ها
ITS, fungal species, morphology, Morus alba, Jiroft
پژوهشگران امیررضا امیرمیجانی، مهدیه صادقیان

چکیده

This study was undertaken to investigate on fungal species associated with dried fruits of Morus alba. During 2016, a total of 25 samples, were collected from common markets in south of Kerman province, Jiroft. After surface disinfestation with 0.5% NaOCl, small fragments of dried fruits were placed onto potato dextrose agar (PDA). Fungal isolates were purified by single spore method. Morphological characteristics such as colony color, shape and size of hypha, conidia and conidiophores were recorded. In addition, for confirmation of morphological identification, genomic DNA was extracted from mycelia by the chelex method. The rDNA ITS region (including 5.8S rDNA) was amplified by a polymerase chain reaction using ITS1 and ITS4 primers. Based on morphological and molecular data, five species including Alternaria alternata, A. tenuissima, Arthrinium arundinis, Cladosporium cladosporioides and Fusarium oxysporum were identified. To the best of our knowledge, this is the first report of fungi associated with dried fruits of white mulberry in Iran.