The genus Fusarium is highly diverse in morphology and have a worldwide distribution. There are several species complexes in Fusarium that are morphologically indistinguishable but phylogenetically clustered in different phylogenetic lineages [1]. During recent years, molecular studies have led to introduction of new species and combinations in the genus Fusarium [2]. This study was carried out for preliminary phylogenetic analysis of some isolates of this genus obtained from greenhouse crops in south of Kerman province. For this regards, 87 sequences of EF1-α region (including 67 sequences from different species of Fusarium and 20 sequences obtained from GeneBank) were used in phylogenetic analysis. The data analyzed using Minimum-Evolution (ME) and Maximum-parsimony (MP) methods in MEGA 7.0 software. According to the results, this genomic region showed suitable resolution for all of the species. However, there are still several isolates of the species complexes such as F. equiseti and F. incarnatum that are phylogenetically clustered in several subclades.