Humans, through their progress and prowess in various scientific fields, have increased their presence on Earth throughout history. This rise in population has become significant in recent decades, leading to an inevitable need to sustain this growing populace. However, numerous risks and limitations exist in this pursuit. Modern science has emphasized the importance of sustainable agricultural systems that align with natural processes, aiming to provide for the current generation while safeguarding the needs of future ones. In developing countries, insect pests pose a serious challenge to agricultural production, reducing crop yields by up to 50%. Pesticide consumption has notably increased, with Iran experiencing a parallel growth in production and pesticide use. The southern agricultural region of Kerman stands as a hub for greenhouse products. Various regional studies have highlighted the adverse effects of pesticide usage on both agricultural workers and consumers. This issue has raised significant health concerns regarding greenhouse products, as well as posing marketing challenges at national and international levels. Upon review, it was noted that biological control methods have been inadequately discussed in the context of greenhouse products. Therefore, the current research aims to assess the knowledge status of greenhouse farmers and their understanding of biological control methods in Jiroft city in 2022. The study involved gathering information from greenhouse owners in the region through random sampling, using face-to-face interviews and questionnaires. Results indicated that the knowledge level of farmers regarding natural enemies of greenhouse pests is notably low (65%), with only two percent demonstrating a very high level of knowledge. Furthermore, the regression model estimation revealed that variables such as literacy level, distance from the city center, and the frequency of interactions with agricultural agents significantly impacted farmers' knowledge. Based on these findings, it is recommended that agricultural organizations introduce and promote the advantages and benefits of biological pest control methods in greenhouses. This could be achieved through training sessions, the establishment of sample greenhouses implementing biological control management systems, and the continuous presentation of local radio programs.