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Title
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Exploring the Interaction Between the Ni(II) Complex based
on Schiff Base ligand and FS-DNA: synthesis,
characterization, X-ray structure determination,
Spectroscopic Methods and Docking Simulation
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Abstract
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A new complex with the formula [NiL2] (a) was synthesized. L is Schiff base
ligand and HL is identified as N-(2-hydroxy-1-naphthylidene)-2-methyl aniline. The
compound was characterized by FT-IR, UV-Vis, elemental analysis, and crystal X-ray
diffraction methods. The X-ray diffraction results confirmed that [NiL2] (a) adopts a
regular square-planar geometry. The interactions of complex (a) with FS-DNA (salmon
sperm DNA) have been studied using UV-Vis, fluorescence spectroscopies and Gel
electrophoresis. The binding constant (Kb), and the apparent bio molecular quenching
constant (kq) for FS-DNA were obtained through Stern-Volmer equation. Thermodynamic
parameters data (∆H°, ΔS° and ΔG°) showed that hydrogen bonding and van der Waals
interactions have an important function in the interaction of the DNA-Ni(II) complex and
the binding mode is the groove binding. This dual finding highlights the complex's
nuanced influence on DNA structure and emphasizes the importance of using multiple
analytical techniques to fully understand metal-DNA interactions.
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