The present study reports the design, synthesis, and characterization of nanoencapsulated Satureja kermanica
essential oil/extract by chitosan biopolymer (SKEO-CSN)/(SKEX-CSN) for the antifungal efficacy against Fusarium oxysporum, Alternaria alternata, Botrytis cinerea, Sclerotinia sclerotiorum, Rhizoctonia solani, and Pythium
aphanidermatum. The prepared SKEO-CSN and SKEX-CSN were characterized by Fourier transform infrared
(FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), thermogravimetric
analysis (TGA), and X-ray diffraction analysis (XRD). GC-Mass analysis was done to identify Satureja kermanica
essential oil chemical compounds (SKEO). Thirty-five different components were detected from GC–MS analysis.
Thymol (46.54 %), and Carvacrol (30.54 %) were demonstrated as major compounds. Antifungal studies showed
that the SKEO-CSN and SKEX-CSN formulation effectively inhibit fungal growth more than free SKEO and SKEX.
According to the results, SKEO-CSN and SKEX-CSN provide a wide range of promising antifungal effects and can
be applied as an efficient green strategy to protect plants from fungus infections