Cutaneous leishmaniasis (CL) is the most common form of the disease which can cause
malignant lesions on the skin. Vaccination for the prevention and treatment of leishmaniasis
can be the most effective way to combat this disease. In this study, we designed a novel
multi-epitope vaccine against Leishmania major (L. major) using immunoinformatics tools to
assess its efficacy in silico. Sequences of Leish-F1 protein (TSA, Leif, and LMSTI1) of L.
major were taken from GenBank. The helper T (Th) and cytotoxic T (Tc) epitopes of the protein were predicted. The final multi-epitope consisted of 18 CTL epitopes joined by AAY
linker. There were also nine HTL epitopes in the structure of the vaccine construct, joined by
GPGPG linker. The profilin adjuvant (the toll-like receptor 11 agonist) was also added into
the construct by AAY Linker. There were 613 residues in the structure of the vaccine construct. The multi-epitope vaccine candidate was stable and non-allergic. The data obtained
from the binding of final multi-epitope vaccine-TLR11 residues (band lengths and weighted
scores) unveiled the ligand and the receptor high score of binding affinity. Moreover, in silico
assessment of the vaccine construct cloning achieved its suitable expression in E. coli host.
Based on these results, the current multi-epitope vaccine prevents L. major infection in silico, while further confirmatory assessments are required.