This study aimed to evaluate the effect of adding molasses in different times on water quality,
growth performance, body biochemical composition, digestive and hepatic enzymes of Nile tilapia
in the biofloc system. Tilapia fingerlings (1.53 ± 0.14 g) were distributed in five treatments
including control, BFT24 (adding molasses to the tanks every 24 h), BFT48 (48 h), BFT72
(72 h), and BFT96 (96 h) and reared for 37 days in fiberglass tanks (130 L), with a stocking density
of one fish per litre. The results showed that highest increases in biomass (740.12 g) and survival
(98.97%) were obtained in BFT24 treatment (P<0.05). The body composition was affected by the
experimental treatments so that the highest protein content was obtained in the BFT72 (P<0.05).
Digestive enzymes activities were significantly (P<0.05) higher in BFT treatments than the control
group. The current study showed higher biomass and survival ratio for Nile tilapia were observed
in BFT24 treatment. The liver and digestive enzymes of Nile tilapia were affected by the different
addition times of molasses to the rearing tanks.