حسین بی باک

The plant of Shirazi thyme with the scientific name of Zartaria multiflora Boiss has been recognized. In this research random amplified polymorphic DNA (RAPD) marker due to high speed and low cost and from inter-simple sequence repeat ( ISSR) because of increasing of correctness were used for surveying genetic variety of 15 masses of thyme from south of Iran. Thyme leaves were collected from 15 different regions of the country. Extraction the DNA from the leaf by cetyltrimethylammonium bromide (CTAB) method with the little change was carried out. The quantity and quality of extracted DNA was measured by electrophoresis equipment & spectrophotometer. From 15markers of RAPD 10 markers and from 13 markers of ISSR 10 markers which had produced the clearer bands in polymerase chain reaction (PCR) were used for analysis. by NTSYS-pc and using of dice similarity coefficient and Unweighted Pair Group Method using arithmetic Averages (UPGMA)related dendrogram was drawn. the cophenetic correlation coefficient was calculated and 2 dimensional graph was shaped based on Principle component analysis (PCA). By considering the results, the markers were produced in the total of 207 bands by percent of polymorphism of 86.9. The size range of bands in markers were variable between 120 up to 3000 bp. 15 selected regions in 3 separated group classified, the established cluster had harmony with geographical conditions. The results of Principle component analysis and the results of cluster analysis were similar. cophenetic correlation coefficient was equal with 74.60%. in this research . the result proved that markers of RAPD & ISSR are useful for specifying the genetic variety and family relationship of this mass.