ناصر عسکری

Lily is the third most important bulb crop in the world with ornamental use as cut flowers and pots. Bulblets of lily are compact and stout, and so they are much better to transfer and to sow than shoots. Common techniques for bulblet propagation are time-consuming, ponderous, and inefficacious and are thus insufficient to market demands. Consideration of the effect of medium type and leaf on bulblet induction and its growth under in vitro conditions was the main purpose of this study. Two types of medium culture based on agar content (liquid [0 g/L] and solid [7 g/L]) were used in this experiment. Also, the medium (MS) was composed supplemented with 3% sucrose (w/v), 0.5 mg/L naphthalene acetic acid (NAA). After surface sterilization of explant of Lilium sp. cv. ‘Santander’, the explants were cultured and were placed at 24˚C of 8/16 h light. Following three months, the number of bulblets per explants, and fresh weight of bulblets and leaf were recorded. The results of this experiment showed that the use of liquid medium culture caused a significant increase in bulblet production. The highest number of bulblets (25) was observed in the explants with leaves cultivated in a liquid medium. Also, this treatment had the highest fresh weight of bulblet (425 mg) and leaf (192.8 mg). In general, owing to cost-effective and increasing yield performance and also the quality of bulblets, these results can be recommended for commercial purposes