Hamidreza Alizadeh

The purpose of this study was to investigate the effects of isolates T22, T9 and T6 of Trichoderma harzianum on isolate F42 of Fusarium oxysporum f. sp. radicis-cucumerinum. The effect of T. harzianum isolates on controlling disease was examined under greenhouse conditions. Results showed that these three isolates, respectively, had the high effect on inhibition of pathogen growth. In examining the severity of disease in the greenhouse, it was found that isolate T22 had the greatest effect on controlling the pathogen. The results of volatile compounds showed that these isolates, respectively, were effective in reducing mycelial growth of isolate F42. The highest peroxidase activity was observed on the fourth day in isolate T6 and the highest phenylalanine ammonia lyase enzyme activity was observed on the fifth day in isolate T22. Based on the results, isolate of T22 showed the greatest effect on the induction of resistance against F42 and may be a successful agent for biological control of root and stem rot of cucumber.