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Abstract
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Walnut has a large number of chromosomes (2n=32), and there have been
very limited cytological studies in this species to date. Since haploid plants have half
the number of the chromosomes of the parents, they will be useful to develop the
karyotype. In our study, haploid plants of ‘Hartley’ obtained via parthenogenesis of
irradiated pollen with 600 Gy were selected. Chromosome number was assessed in
root tip cells of in vitro haploid plants, using the standard Feulgen technique. Micro
Measure v3.3 software was used for chromosomal analyses. Length of the
16 chromosomes varied from 1.55 to 3.53 μm. Centromere position of chromosomes
were measured according to Levan et al. (1964). The results showed that among the
16 chromosomes, 10 of them were metacentric, 5 submetacentric and
1 subtelocentric.
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