|
Abstract
|
Extensive samplings were conducted on rice fields infected by agent of rice crown rot in Mazandaran Province as
well as bean farms infected by anthracnose in Zanjan province, Iran, during 2014. Fungal isolations were conducted
using single spore method. During isolation of rice crown rot and anthracnose agents, some other fungal isolates were
grown on water agar (WA). Fungal species identification was performed using morphological features and molecular
data (ITS regions were amplified by ITS1 and ITS4 primers). Among the fungi, also Volutella ciliata (on bean
sheath) Asodesmis microscopica and Westerdykella purpurea (on rice root) were identified. Colony of V. ciliate on
PDA, reaching 9.1 cm after 7 days, surfa
-septate, ellipsoid,
Ascodesmis microscopica On PDA, fully covered the petri after seven days at 25oC in
darkness. surface and reverse cream. Conidiomata apothecial, Asci clavate with eight ascospores. Ascospores
Spherical to ellipsoidal, smooth with age verruculose, 8-11× 10-14 µm. Westerdykella purpurea Colonies appressed,
cream on oatmeal agar; attaining 4.3 cm diam after 7 d at 25 °C, producing distinct, brownish black cleistothecia,
containing globose to subglobose asci (12 18 × 11
ascospores. According to the knowledge, Volutella ciliata Ascodesmis microscopica and Westerdykella purpurea
are three new taxa for mycobiota of Iran.
|