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Abstract
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Tomato leaf curl Palampur virus (ToLCPalV, Begomovirus solanumpalampurense, family Geminiviridae) is a highly destructive
virus affecting tomato and cucurbits crops in Iran. In this study, we report the first detection of tomato leaf curl betasatellite
(ToLCB, Betasatellite solani) in association with ToLCPalV in zucchini plants showing severe disease symptoms in Anbar-Abad,
southeastern Iran. Using agroinoculation experiments on zucchini plants with different combinations of ToLCPalV genomic
components and ToLCB, we found that plants co-inoculated
with DNA-A
+ DNA-B
+ ToLCB exhibited more severe symptoms
compared to those inoculated with DNA-A
+ DNA-B
or DNA-A
+ ToLCB. These results suggest that the severe symptoms are due
to interactions between ToLCPalV DNA-B
and ToLCB. Although both DNA-A
+ DNA-B
and DNA-A
+ ToLCB induced disease
symptoms individually, the resultant symptoms were different between the two treatments. This is the first report of the association
of a bipartite begomovirus with a betasatellite in Iran. Given the widespread occurrence of ToLCPalV and the association of
ToLCB with different mono-and
bipartite geminiviruses including its recent association with a turncurtovirus, further studies
are warranted to investigate interaction between ToLCB and mono-or
bipartite geminiviruses in southeastern Iran.
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